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DGE Analysis on S. mediterranea

 
Taking advantage of digital gene expression (DGE) sequencing technology we compare all the available transcriptomes for S. mediterranea and improve their annotation. These results are accessible via web for the community of researchers.
Using the quantitative nature of DGE, we describe the transcriptional profile of neoblasts and present 42 new neoblast genes, including several cancer-related genes and transcription factors. Furthermore, we describe in detail the Smed-meis-like gene and the three Nuclear Factor Y subunits Smed-nf-YA, Smed-nf-YB-2 and Smed-nf-YC.
In conclusion, we found that DGE is a valuable tool in our case for gene discovery, quantification and annotation. The application of DGE in S. mediterranea confirms the planarian stem cells or neoblasts as a complex population of pluripotent and multipotent cells regulated by a mixture of transcription factors and cancer-related genes.

 

 

Data Tables

Transcriptomes relationship

Use the buttons below to access to the tables listing the correspondence between all the publicly available transcriptomes of S. mediterranea. Percentages refer to the minimum coverage cut-off:

 

Expression level

Follow the links on this section to access to the tables listing the expression levels corresponding to the transcripts of each dataset:

Publicly available transcriptomes:

 
S. mediterranea ESTs:

 
Genome assembly AUVC01 repeat masked:

 

Dotplot Overview of p-values

 

ALL (n=98156)
FILTERED (n=33455)

 


Page last modified on Thursday 07 of May, 2015 15:29:59 UTC

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